A Texas mother of five’s desperate last stand against the twisted sisters accused of hacking her to death was captured in a chilling new photo of the bloody attack in a dusty border town.

The haunting image — apparently snapped moments after 19-year-old Amaya Cookie Diaz allegedly plunged a knife into Caroline “Caro” Peña’s back – shows the 32-year-old victim’s pink T-shirt soaked in blood.

Just feet away, three women can be seen standing in the grass beside the visibly wounded Peña – including two whose clothing matches descriptions police later gave of the assailants.

Surveillance footage later obtained by cops showed Cookie clad in a red tank top and blue jeans, while her older sister, 21-year-old Kitty Mia Diaz – identifiable by tattoos covering her right arm – was seen wearing a pink tank top paired with blue jeans, according to a criminal complaint filed in the case.

It wasn’t immediately clear whether the third woman in the disturbing photo – seen dressed in a gray T-shirt – is 21-year-old Kyandra Renee Faz, who was also charged in Peña’s murder.

In the image, a man appears to have his arms wrapped around Cookie’s shoulders while Peña is seen facing and speaking to another man – possibly her nephew, who reportedly drove her from the grisly scene to a local hospital.

The chilling photo was uploaded to a local community Facebook page, three sources confirmed to The Post, but swiftly scrubbed from the platform after Peña succumbed to the knife wounds at a hospital in San Antonio at around 9 p.m. Thursday.

Still, it shows that Peña “was a fighter,” her longtime friend, Zelina Ochoa, previously told The Post.

“She had just collapsed and she stood right back up,” Ochoa said.

“It’s a very powerful picture because it shows, you know, these three girls showed up with their weapons and Caro showed up with nothing but her hands,” she added.

Peña knew her accused killers, Del Rio Police Chief Frank Ramirez revealed Tuesday.

The nature of their relationship remains unclear

Police have not yet offered a motive for the alleged attack, which remains under investigation, a department spokesperson told The Post.

Faz told investigators that Peña had “arrived at her residence looking to start a fight” – but was soon met by the sisters, who drove up to the home in a black Chrysler 300, the complaint states.

The surveillance clip shows Cookie fly out of the vehicle’s passenger seat and confront Peña — while holding an object believed by cops to be a knife in her right hand, according to the court document.

Cookie is then seen in the clip striking the mother in the back, where blood begins soaking her shirt, the doc states.

That’s when Kitty and Faz jumped in — allegedly beating Peña before running away, the complaint continues.

Just two hours after the brutal attack, Cookie and Kitty flashed twisted smiles as police hauled them off in handcuffs.

Footage taken outside the siblings’ home showed a barefoot Kitty – wearing tight black shorts and a halter top with an illustration of white hands cupping her breasts – grinning briefly at the ground as two officers escorted her into a patrol car around 4 p.m. Thursday.

Her similarly scantily clad younger sister appeared to put on a show for the camera, brazenly flashing her pearly whites and giggling after sarcastically yelling at the man behind the camera, “Stop recording!”

Faz and the Diaz sisters are behind bars after each were slapped with $5 million bonds by a judge during their first court appearance Friday, when they all requested court-appointed attorneys.

They’re each charged with first-degree murder.